Synthesis in vitro and translocation of apolipoprotein AI across microsomal vesicles.
نویسندگان
چکیده
Apolipoprotein AI of rats has been synthesized in a cell-free wheat germ system and cotranslationally translocated into dog pancreas microsomal vesicles. Translocation is accompanied by cleavage of a signal sequence of 18 amino acid residues and is dependent on the recently purified signal recognition protein.
منابع مشابه
In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-alpha-factor.
The in vitro synthesized precursor of the alpha-factor pheromone, prepro-alpha-factor, of Saccharomyces cerevisiae was translocated across yeast microsomal membranes in either a homologous or a wheat germ cell free system. Translocated prepro-alpha-factor was glycosylated, sedimented with yeast microsomal vesicles, and was protected from digestion by added protease, but was soluble after alkali...
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Translocation-competent microsomal membrane vesicles of dog pancreas were shown to selectively bind nascent, in vitro assembled polysomes synthesizing secretory protein (bovine prolactin) but not those synthesizing cytoplasmic protein (alpha and beta chain of rabbit globin). This selective polysome binding capacity was abolished when the microsomal vesicles were salt-extracted but was restored ...
متن کاملTranslocation of proteins across the endoplasmic reticulum.
Translocation-competent microsomal membrane vesicles of dog pancreas were shown to selectively bind nascent, in vitro assembled polysomes synthesizing secretory protein (bovine prolactin) but not those synthesizing cytoplasmic protein (alpha and beta chain of rabbit globin) . This selective polysome binding capacity was abolished when the microsomal vesicles were salt-extracted but was restored...
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Radiolabeled UDPGlc incubated with rough endoplasmic reticulum (RER)-derived microsomes from rat liver became associated with the vesicles. This microsomal uptake of nucleotide sugar was time and temperature dependent. Analysis of the molecular species containing radiolabel revealed that initial uptake represented entry of predominantly intact UDPGlc in the microsomes. Conclusive evidence for p...
متن کاملPreparation of microsomal membranes for cotranslational protein translocation.
that incorporation of radioactivity into hot trichloroacetic acid-insoluble material continues for at least 15 min and then levels off. 6 The most difficult step in this procedure is the isolation of highly purified mRNA. The lipoprotein mRNA is the only mRNA of E. coli that has been purified to homogeneity. 39 When the unique features of an mRNA cannot be used to facilitate its isolation from ...
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ورودعنوان ژورنال:
- European journal of biochemistry
دوره 120 3 شماره
صفحات -
تاریخ انتشار 1981